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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HTRA1 Polyclonal antibody (29618-1-AP)

    WB analysis using 29618-1-AP (same clone as 29618-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29618-1-AP (HTRA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29618-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human placenta tissue using HTRA1 Polyclonal antibody (29618-1-AP)

    IP experiment of human placenta using 29618-1-AP (same clone as 29618-1-PBS)

    IP result of anti-HTRA1 (IP:29618-1-AP, 4ug; Detection:29618-1-AP 1:500) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 29618-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HTRA1 Polyclonal antibody (29618-1-AP)

    IHC staining of human skin cancer using 29618-1-AP (same clone as 29618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29618-1-AP (HTRA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29618-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HTRA1 Polyclonal antibody (29618-1-AP)

    IF Staining of HepG2 using 29618-1-AP (same clone as 29618-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HTRA1 antibody (29618-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29618-1-PBS in a different storage buffer formulation.

HTRA1 Polyclonal antibody, PBS Only

HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 29618-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.4.21.-, HtrA, PRSS11

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29618-1-PBS targets HTRA1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31194

Product name: Recombinant human HTRA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 358-480 aa of NM_002775

Sequence: DKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP

相同性解析による交差性が予測される生物種
Full Name HtrA serine peptidase 1
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberNM_002775
Gene Symbol HTRA1
Gene ID (NCBI) 5654
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92743
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 51 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

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