Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spleen tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
13830-1-AP targets HTR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4742 Product name: Recombinant human HTR7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 263-445 aa of BC047526 Sequence: YKAARKSAAKHKFPGFPRVEPDSVIALNGIVKLQKEVEECANLSRLLKHERKNISIFKREQKAATTLGIIVGAFTVCWLPFFLLSTARPFICGTSCSCIPLWVERTFLWLGYANSLINPFIYAFFNRDLRTTYRSLLQCQYRNINRKLSAAGMHEALKLAERPERPEFVLQNADYCRKKGHDS 相同性解析による交差性が予測される生物種 |
| Full Name | 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) |
| Calculated molecular weight | 445 aa, 49 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC047526 |
| Gene Symbol | HTR7 |
| Gene ID (NCBI) | 3363 |
| RRID | AB_2122706 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34969 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Serotonin (5-hydroxytryptamine; 5-HT) acts as a neurotransmitter and neuromodulator in the central nervous system(CNS) (PMID:23100172). The behavioral effects of 5-HT are mediated by a large family of specific 5-HT receptors. HTR7 (5-hydroxytryptamine receptor 7) is a G protein-coupled receptor (GPCR), and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (PMID:34164474). It is located in different brain regions, such as the hypothalamus, thalamus, cerebral cortex, and hippocampus (PMID:29637287). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (PMID:9154233).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HTR7 antibody 13830-1-AP | Download protocol |
| WB protocol for HTR7 antibody 13830-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Biomedicines T Lymphocyte Serotonin 5-HT7 Receptor Is Dysregulated in Natalizumab-Treated Multiple Sclerosis Patients | ||
Onco Targets Ther 5-HT7 Receptor Contributes to Proliferation, Migration and Invasion in NSCLC Cells. | ||
Cancer Lett Serotonylation in tumor-associated fibroblasts contributes to the tumor-promoting roles of serotonin in colorectal cancer |
