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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPH1 Monoclonal antibody (66723-1-Ig)

    WB analysis using 66723-1-Ig (same clone as 66723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66723-1-Ig (HSPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSPH1 Monoclonal antibody (66723-1-Ig)

    IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSPH1 Monoclonal antibody (66723-1-Ig)

    IHC staining of mouse testis using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66723-1-Ig (HSPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSPH1 Monoclonal antibody (66723-1-Ig)

    IF Staining of HeLa using 66723-1-Ig (same clone as 66723-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66723-1-Ig (HSPH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66723-1-PBS in a different storage buffer formulation.

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HSPH1 Monoclonal antibody, PBS Only

HSPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66723-1-PBS
Clone No.3H5A5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Antigen NY CO 25, DKFZp686M05240, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66723-1-PBS targets HSPH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSPH1 fusion protein Ag27521 相同性解析による交差性が予測される生物種
Full Name heat shock 105kDa/110kDa protein 1
Calculated molecular weight 858 aa, 97 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC037553
Gene Symbol HSPH1
Gene ID (NCBI) 10808
RRIDAB_2882074
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92598
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. Western blot analysis using this antibody detected the phosphorylated protein around 110 kDa in various lysates.