Validation Data Gallery

Filter: AllWBIHC

WB analysis of MCF-7 using 13383-1-AP (same clone as 13383-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 13383-1-AP (same clone as 13383-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 13383-1-AP (same clone as 13383-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 13383-1-AP (same clone as 13383-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13383-1-AP (same clone as 13383-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13383-1-PBS in a different storage buffer formulation.

HSPH1 Polyclonal antibody, PBS Only

HSPH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13383-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0201, HSP110, HSP105, Heat shock protein family H member 1, Antigen NY-CO-25

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13383-1-PBS targets HSPH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4224 Product name: Recombinant human HSPH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 508-858 aa of BC037553 Sequence: MSSEADMECLNQRPPENPDTDKNVQQDNSEAGTQPQVQTDAQQTSQSPPSPELTSEENKIPDADKANEKKVDQPPEAKKPKIKVVNVELPIEANLVWQLGKDLLNMYIETEGKMIMQDKLEKERNDAKNAVEEYVYEFRDKLCGPYEKFICEQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD 相同性解析による交差性が予測される生物種
Full Name	heat shock 105kDa/110kDa protein 1
Calculated molecular weight	858 aa, 97 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC037553
Gene Symbol	HSPH1
Gene ID (NCBI)	10808
RRID	AB_2233157
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92598
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. This antibody recognizes both HSP105α and HSP105β isoforms. Western blot analysis using this antibody detected a major band around 100-110 kDa in Jurkat cells.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 13383-1-AP (same clone as 13383-1-PBS)

WB analysis of Jurkat using 13383-1-AP (same clone as 13383-1-PBS)

WB analysis of human brain using 13383-1-AP (same clone as 13383-1-PBS)

WB analysis of K-562 using 13383-1-AP (same clone as 13383-1-PBS)

WB analysis of HeLa using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human testis using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human pancreas cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human colon cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

IHC staining of human liver cancer using 13383-1-AP (same clone as 13383-1-PBS)

HSPH1 Polyclonal antibody, PBS Only

HSPH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information