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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP60 Recombinant monoclonal antibody (86275-1-RR)

    WB analysis using 86275-1-RR (same clone as 86275-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86275-1-RR (HSP60 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSP60 Recombinant monoclonal antibody (86275-1-RR)

    WB analysis of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    WB result of HSP60 antibody (86275-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP60 transfected HeLa cells. This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP60 Recombinant monoclonal antibody (86275-1-RR)

    IF Staining of HeLa using 86275-1-RR (same clone as 86275-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP60 antibody (86275-1-RR, Clone: 250248D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86275-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86275-1-RR

    Affinity and Kinetic Characterization of 86275-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86275-1-RR against Human HSPD1 were performed. The affinity constant is 7.43 nM.

HSP60 Recombinant monoclonal antibody, PBS Only

HSP60 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86275-1-PBS
Clone No.250248D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

HSPD1, 60 kDa chaperonin, Chaperonin 60, CPN60, EC:5.6.1.7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86275-1-PBS targets HSP60 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7401

Product name: Recombinant human HSPD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 225-573 aa of BC003030

Sequence: SPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

相同性解析による交差性が予測される生物種
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene Symbol HSP60
Gene ID (NCBI) 3329
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10809
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

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