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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP75 Polyclonal antibody (14887-1-AP)

    WB analysis using 14887-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GRP75 Polyclonal antibody (14887-1-AP)

    WB analysis of A549 cells using 14887-1-AP

    A549 cells (shcontrol and shRNA of GRP75)were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:500.

  • Western Blot (WB) analysis of mouse testis tissue using GRP75 Polyclonal antibody (14887-1-AP)

    WB analysis of mouse testis using 14887-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GRP75 Polyclonal antibody (14887-1-AP)

    IP experiment of mouse brain using 14887-1-AP

    IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:2000) with mouse brain tissue lysate 3680ug.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using GRP75 Polyclonal antibody (14887-1-AP)

    IP experiment of NIH/3T3 using 14887-1-AP

    IP result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using GRP75 Polyclonal antibody (14887-1-AP)

    IHC staining of human colon using 14887-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14887-1-AP (GRP75 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRP75 Polyclonal antibody (14887-1-AP)

    IF Staining of HepG2 using 14887-1-AP

    Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRP75 Polyclonal antibody (14887-1-AP)

    IF Staining of HepG2 using 14887-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP75 antibody (14887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using GRP75 Polyclonal antibody (14887-1-AP)

    FC experiment of HepG2 using 14887-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GRP75 Polyclonal antibody (14887-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

GRP75 Polyclonal antibody

GRP75 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 14887-1-AP
Publications(82)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-29 cells, A549 cells, mouse testis tissue, Jurkat cells, NIH/3T3 cells, mouse liver
Positive IP detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14887-1-AP targets GRP75 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6673

Product name: Recombinant human GRP75 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-667 aa of BC000478

Sequence: YLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGT

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 9 (mortalin)
Calculated molecular weight 74 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
RRIDAB_2120458
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP38646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

Protocols

Product Specific Protocols
FC protocol for GRP75 antibody 14887-1-APDownload protocol
IF protocol for GRP75 antibody 14887-1-APDownload protocol
IHC protocol for GRP75 antibody 14887-1-APDownload protocol
IP protocol for GRP75 antibody 14887-1-APDownload protocol
WB protocol for GRP75 antibody 14887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IP,IHC

Signal Transduct Target Ther

GRP75 triggers white adipose tissue browning to promote cancer-associated cachexia

Authors - Xu Chen
View Article
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
View Article

Cell Metab

Gut microbiota-derived lysine phenylacetylation impairs mitochondrial function and is alleviated by SIRT3.

Authors - Wei Du
View Article

Nat Metab

LONP1 regulation of mitochondrial protein folding provides insight into beta cell failure in type 2 diabetes

Authors - Jin Li
View Article
humanWB,IHC

Nat Cell Biol

The mitochondria enzyme OGDH defends against disulfidptosis by licensing METTL3-regulated NRF2 translation

Authors - Xiao-Yan Chen
View Article
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
View Article
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