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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis using 67173-1-Ig (same clone as 67173-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of HEK-293 using 67173-1-Ig (same clone as 67173-1-PBS)

    WB result of HSPA4 antibody (67173-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells. This data was developed using the same antibody clone with 67173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of Jurkat using 67173-1-Ig (same clone as 67173-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67173-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using HSPA4 Monoclonal antibody (67173-1-Ig)

    WB analysis of pig brain using 67173-1-Ig (same clone as 67173-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67173-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HSPA4 Monoclonal antibody, PBS Only

HSPA4 Monoclonal Antibody for WB, ELISA
Cat No. 67173-1-PBS
Clone No.2F2C12

Host / Isotype

Mouse / IgM

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

2F2C12, APG2, Heat shock 70 kDa protein 4, Heat shock 70-related protein APG-2, Heat shock protein family H member 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67173-1-PBS targets HSPA4 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16624

Product name: Recombinant human HSPA4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 680-840 aa of BC110861

Sequence: NLGQPIKIRFQESEERPKLFEELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 4
Calculated molecular weight 840 aa, 94 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC110861
Gene Symbol HSPA4
Gene ID (NCBI) 3308
RRIDAB_2882469
Conjugate Unconjugated
Form
FormLiquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP34932
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors.

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