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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA1B Polyclonal antibody (25405-1-AP)

    WB analysis using 25405-1-AP (same clone as 25405-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using HSPA1B Polyclonal antibody (25405-1-AP)

    WB analysis of rat brain using 25405-1-AP (same clone as 25405-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using HSPA1B Polyclonal antibody (25405-1-AP)

    WB analysis of mouse brain using 25405-1-AP (same clone as 25405-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HSPA1B Polyclonal antibody (25405-1-AP)

    IP experiment of HEK-293 using 25405-1-AP (same clone as 25405-1-PBS)

    IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:5000) with HEK-293 cells lysate 2320 ug. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using HSPA1B Polyclonal antibody (25405-1-AP)

    IP experiment of K-562 using 25405-1-AP (same clone as 25405-1-PBS)

    IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HSPA1B Polyclonal antibody (25405-1-AP)

    IHC staining of human tonsillitis using 25405-1-AP (same clone as 25405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25405-1-AP (HSP70 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HSPA1B Polyclonal antibody (25405-1-AP)

    IHC staining of human stomach using 25405-1-AP (same clone as 25405-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25405-1-AP (HSPA1B antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSPA1B Polyclonal antibody (25405-1-AP)

    IF Staining of HeLa using 25405-1-AP (same clone as 25405-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HSPA1B antibody (25405-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

HSPA1B Polyclonal antibody, PBS Only

HSPA1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25405-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

HSP70, HSP72

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25405-1-PBS targets HSPA1B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13700

Product name: Recombinant human HSPA1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 292-641 aa of BC057397

Sequence: DFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1B
Calculated molecular weight 70 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC057397
Gene Symbol HSP70
Gene ID (NCBI) 3304
RRIDAB_2880063
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP08107
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

1. What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.


2. FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

a. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.


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