GRP94 Monoclonal antibody
GRP94 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgM
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1H10B7
Cat no : 60012-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Human heart, human heart tissue, human liver tissue, human lung tissue, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, Hela cells, MCF-7 cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
60012-1-Ig targets GRP94 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC009195 |
| Gene symbol | HSP90B1 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2119056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRP94 antibody 60012-1-Ig | Download protocol |
| IHC protocol for GRP94 antibody 60012-1-Ig | Download protocol |
| IF protocol for GRP94 antibody 60012-1-Ig | Download protocol |
| FC protocol for GRP94 antibody 60012-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Influenza M2 protein regulates MAVS-mediated signaling pathway through interacting with MAVS and increasing ROS production. | ||
Front Cell Dev Biol Extracellular Vesicles Carry lncRNA SNHG16 to Promote Metastasis of Breast Cancer Cells via the miR-892b/PPAPDC1A Axis. | ||
Neurochem Res Novel Role of HAX-1 in Neurons Protection After Spinal Cord Injury Involvement of IRE-1. | ||
J Adv Res Development of a novel thymidylate synthase (TS) inhibitor capable of up-regulating P53 expression and inhibiting angiogenesis in NSCLC. | ||
Front Physiol Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study. |
