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- KD/KO Validated
GRP94 Polyclonal antibody
GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10979-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, A549 cells, K-562 cells, NIH/3T3 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse pancreas tissue, MCF-7 cells, HEK-293 cells, human appendicitis tissue |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 7 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
10979-1-AP targets GRP94 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種 |
Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
Calculated molecular weight | 96 kDa |
Observed molecular weight | 94 kDa |
GenBank accession number | BC009195 |
Gene symbol | HSP90B1 |
Gene ID (NCBI) | 7184 |
RRID | AB_2119057 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GRP94 antibody 10979-1-AP | Download protocol |
IHC protocol for GRP94 antibody 10979-1-AP | Download protocol |
IF protocol for GRP94 antibody 10979-1-AP | Download protocol |
IP protocol for GRP94 antibody 10979-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Neuroinflammation TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease. | ||
Vet Microbiol ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. | ||
Mol Med Rep Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats. | ||
J Immunol Res Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis | ||
Carcinogenesis Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis | ||
Exp Brain Res CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis |