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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP10 Polyclonal antibody (16512-1-AP)

    WB analysis using 16512-1-AP (same clone as 16512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16512-1-AP (HSP10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP10 Polyclonal antibody (16512-1-AP)

    IHC staining of human lung cancer using 16512-1-AP (same clone as 16512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSP10 Polyclonal antibody (16512-1-AP)

    IHC staining of human lung cancer using 16512-1-AP (same clone as 16512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using HSP10 Polyclonal antibody (16512-1-AP)

    IHC staining of mouse adrenal gland using 16512-1-AP (same clone as 16512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16512-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using HSP10 Polyclonal antibody (16512-1-AP)

    IHC staining of mouse adrenal gland using 16512-1-AP (same clone as 16512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 16512-1-AP (HSP10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16512-1-PBS in a different storage buffer formulation.

HSP10 Polyclonal antibody, PBS Only

HSP10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16512-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

HSPE1, 10 kDa chaperonin, CPN10, Early-pregnancy factor, Heat shock protein family E member 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16512-1-PBS targets HSP10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9732

Product name: Recombinant human HSPE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-102 aa of BC023518

Sequence: MAGQAFRKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD

相同性解析による交差性が予測される生物種
Full Name heat shock 10kDa protein 1 (chaperonin 10)
Calculated molecular weight 102 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC023518
Gene Symbol HSP10
Gene ID (NCBI) 3336
RRIDAB_3085493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61604
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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