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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSL Polyclonal antibody (17333-1-AP)

    WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Polyclonal antibody (17333-1-AP)

    IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using HSL Polyclonal antibody (17333-1-AP)

    IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Polyclonal antibody (17333-1-AP)

    IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSL Polyclonal antibody (17333-1-AP)

    IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

HSL Polyclonal antibody, PBS Only

HSL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17333-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17333-1-PBS targets HSL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11360

Product name: Recombinant human LIPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 728-1076 aa of BC070041

Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

相同性解析による交差性が予測される生物種
Full Name lipase, hormone-sensitive
Calculated molecular weight 117 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC070041
Gene Symbol HSL
Gene ID (NCBI) 3991
RRIDAB_2878386
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05469
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.

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