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Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    WB analysis of human testis using 11970-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    WB analysis of human testis using 11970-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    WB analysis of human testis using 11970-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using HSFY1 Polyclonal antibody (11970-1-AP)

    IHC staining of human testis using 11970-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HSFY1 Polyclonal antibody

HSFY1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11970-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSFY1 fusion protein Ag2592 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor, Y-linked 1
Calculated molecular weight 401 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC055414
Gene Symbol HSFY1
Gene ID (NCBI) 86614
RRIDAB_2877810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LI6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.

Protocols

Product Specific Protocols
WB protocol for HSFY1 antibody 11970-1-APDownload protocol
IHC protocol for HSFY1 antibody 11970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols