Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32594-1-AP targets HSF2BP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37637 Product name: Recombinant human HSF2BP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC000153 Sequence: MGEAGAAEEACRHMGTKEEFVKVRKKDLERLTTEVMQIRDFLPRILNGEVLESFQKLKIVEKNLERKEQELEQLKMDCEHFKARLETVQADNIREKKEKLALRQQLNEAKQQLLQQAEYCTEMGAAACTLLWGVSSSEEVVKAILGGDKALKFFSITGQTMESFVKSLDGDVQELDSDESQFVFALAGIVTNVAAIACGREFLVNSSRVLLDTILQLLGDLKPGQCTKLKVLMLMSLYNVSINLKGLKYISESPGFIPLLWWLLSDPDAEVCLHVLRLVQSVVLEPEVFSKSASEFRSSLPLQRILAMSKSRNPRLQTAAQELLEDLRTLEHNV 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock transcription factor 2 binding protein |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC000153 |
| Gene Symbol | HSF2BP |
| Gene ID (NCBI) | 11077 |
| RRID | AB_3742668 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heat shock factor 2-binding protein (HSF2BP, also known as MEILB2) is a binding protein of HSF2, which is involved in modulating HSF2 activation in testis (PMID: 9651507). Naturally occurring elevated production of HSF2BP in tumors may be a source of cancer-promoting genomic instability and a vulnerable target (PMID: 31960047; 34790765).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HSF2BP antibody 32594-1-AP | Download protocol |
| WB protocol for HSF2BP antibody 32594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |