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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD3B2 Monoclonal antibody (67572-1-Ig)

    WB analysis using 67572-1-Ig (same clone as 67572-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using HSD3B2 Monoclonal antibody (67572-1-Ig)

    WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using HSD3B2 Monoclonal antibody (67572-1-Ig)

    WB analysis of human placenta using 67572-1-Ig (same clone as 67572-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using HSD3B2 Monoclonal antibody (67572-1-Ig)

    WB analysis of human adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig adrenal gland tissue using HSD3B2 Monoclonal antibody (67572-1-Ig)

    WB analysis of pig adrenal gland using 67572-1-Ig (same clone as 67572-1-PBS)

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67572-1-Ig (HSD3B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HSD3B2 Monoclonal antibody (67572-1-Ig)

    IF Staining of A549 using 67572-1-Ig (same clone as 67572-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HSD3B2 antibody (67572-1-Ig, Clone: 2E7B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67572-1-PBS in a different storage buffer formulation.

HSD3B2 Monoclonal antibody, PBS Only

HSD3B2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67572-1-PBS
Clone No.2E7B4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

2E7B4, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3b HSD, 3-beta-HSD adrenal and gonadal type

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67572-1-PBS targets HSD3B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4291

Product name:

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC038419
Gene Symbol HSD3B2
Gene ID (NCBI) 3284
RRIDAB_2882785
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP26439
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in the placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).