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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B6 Polyclonal antibody (11855-1-AP)

    WB analysis using 11855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    WB analysis of human liver using 11855-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B6 Polyclonal antibody (11855-1-AP)

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

HSD17B6 Polyclonal antibody

HSD17B6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11855-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman lung cancer tissue, human prostate hyperplasia tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11855-1-AP targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2427

Product name: Recombinant human HSD17B6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-317 aa of BC020710

Sequence: MWLYLAAFVGLYYLLHWYRERQVVSHLQDKYVFITGCDSGFGNLLARQLDARGLRVLAACLTEKGAEQLRGQTSDRLETVTLDVTKMESIAAATQWVKEHVGDRGLWGLVNNAGILTPITLCEWLNTEDSMNMLKVNLIGVIQVTLSMLPLVRRARGRIVNVSSILGRVAFFVGGYCVSKYGVEAFSDILRREIQHFGVKISIVEPGYFRTGMTNMTQSLERMKQSWKEAPKHIKETYGQQYFDALYNIMKEGLLNCSTNLNLVTDCMEHALTSVHPRTRYSAGWDAKFFFIPLSYLPTSLADYILTRSWPKPAQAV

相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
Calculated molecular weight 317 aa, 36 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC020710
Gene Symbol HSD17B6
Gene ID (NCBI) 8630
RRIDAB_2295487
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14756
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B6 antibody 11855-1-APDownload protocol
IHC protocol for HSD17B6 antibody 11855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Breast Cancer Res Treat

The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer.

Authors - Keely May McNamara
View Article
humanWB

Cancer Cell Int

Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma.

Authors - Lei Lv
View Article
humanWB

Cell Death Discov

HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma

Authors - Tian Tian
View Article
mouseWB

Toxicol Lett

Metabolomics reveals dysregulated all-trans retinoic acid and polyunsaturated fatty acid metabolism contribute to PXR-induced hepatic steatosis in mice

Authors - Liang Yan
View Article