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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B6 Polyclonal antibody

HSD17B6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11855-1-AP

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

Validation Data Gallery

Applications:
  • WB analysis using 11855-1-AP

    WB analysis using 11855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 11855-1-AP

    WB analysis of human liver using 11855-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 11855-1-AP

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11855-1-AP

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11855-1-AP

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11855-1-AP

    IHC staining of human lung cancer using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate hyperplasia using 11855-1-AP

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 11855-1-AP

    IHC staining of human prostate hyperplasia using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver using 11855-1-AP

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 11855-1-AP

    IHC staining of human liver using 11855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman lung cancer tissue, human prostate hyperplasia tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11855-1-AP targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B6 fusion protein Ag2427 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
Calculated molecular weight 317 aa, 36 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC020710
Gene symbol HSD17B6
Gene ID (NCBI) 8630
RRIDAB_2295487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B6 antibody 11855-1-APDownload protocol
IHC protocol for HSD17B6 antibody 11855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Breast Cancer Res Treat

The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer.

Authors - Keely May McNamara
View Article
humanWB

Cancer Cell Int

Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma.

Authors - Lei Lv
View Article
humanWB

Cell Death Discov

HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma

Authors - Tian Tian
View Article