Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, human placenta tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IHC | See 22 publications below |
Product Information
10978-1-AP targets HSD17B2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1411 Product name: Recombinant human HSD17B2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC009581 Sequence: MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYTYLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDITKPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKSKGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLEKDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICLAHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxysteroid (17-beta) dehydrogenase 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC009581 |
| Gene Symbol | HSD17B2 |
| Gene ID (NCBI) | 3294 |
| RRID | AB_2279863 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| WB protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Hypoxia-Reoxygenation Couples 3βHSD1 Enzyme and Cofactor Upregulation to Facilitate Androgen Biosynthesis and Hormone Therapy Resistance in Prostate Cancer | ||
Phytomedicine Paridis rhizoma total saponins ameliorate abnormal uterine bleeding via regulating Proteus mirabilis 2024-mediated estrogen metabolism in the gut-reproductive axis. | ||
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
J Hazard Mater Exposure to 1-nitropyrene after weaning induces anxiety-like behavior partially by inhibiting steroid hormone synthesis in prefrontal cortex | ||
J Transl Med Intratumoral localization and activity of 17beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer: a potent prognostic factor. | ||
Fertil Steril Differential expression of steroidogenic enzymes according to endometriosis type. |