Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, HuH-7 cells, MDA-MB-231 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31463-1-AP targets HSD17B12 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSD17B12 fusion protein Ag34833 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxysteroid (17-beta) dehydrogenase 12 |
| Calculated molecular weight | 312 aa, 34 kDa |
| Observed molecular weight | ~34 kDa |
| GenBank accession number | BC012043 |
| Gene Symbol | HSD17B12 |
| Gene ID (NCBI) | 51144 |
| RRID | AB_3669997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q53GQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSD17B12 (17β-hydroxysteroid dehydrogenase 12) is an important protein belonging to the hydroxysteroid dehydrogenase family, which is mainly involved in the metabolism of steroids and fatty acids in the body. It has diverse functions in cells and is involved in various biochemical reactions, especially playing a key role in sex hormone synthesis and fatty acid elongation. HSD17B12 can convert estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. HSD17B12 is expressed in a variety of tissues, especially in the brain tissue and the digestive system. In disease states, such as colorectal cancer (CRC), the expression level of HSD17B12 is significantly increased, suggesting that it may play a role in the occurrence and development of tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD17B12 antibody 31463-1-AP | Download protocol |
| IHC protocol for HSD17B12 antibody 31463-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |