Validation Data Gallery

Filter: AllWBIHC

WB analysis of L02 using 16303-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16303-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16303-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16303-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16303-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16303-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary using 16303-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 16303-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary cancer using 16303-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16303-1-AP (HSD17B11 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSD17B11 Polyclonal antibody

HSD17B11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16303-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17-beta-HSD XI, 17-beta-HSD 11, 17 BETA HSDXI, 17 BETA HSD11, 17 beta HSD XI

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	L02 cells, mouse heart tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected in	human ovary cancer tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

16303-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9278 Product name: Recombinant human HSD17B11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-300 aa of BC021673 Sequence: SVTGEIVLITGAGHGIGRLTAYEFAKLKSKLVLWDINKHGLEETAAKCKGLGAKVHTFVVDCSNREDIYSSAKKVKAEIGDVSILVNNAGVVYTSDLFATQDPQIEKTFEVNVLAHFWTTKAFLPAMTKNNHGHIVTVASAAGHVSVPFLLAYCSSKFAAVGFHKTLTDELAALQITGVKTTCLCPNFVNTGFIKNPSTSLGPTLEPEEVVNRLMHGILTEQKMIFIPSSIAFLTTLERILPERFLAVLKRKISVKFDAVIGYKMKAQ 相同性解析による交差性が予測される生物種
Full Name	hydroxysteroid (17-beta) dehydrogenase 11
Calculated molecular weight	300 aa, 33 kDa
Observed molecular weight	28-33 kDa
GenBank accession number	BC021673
Gene Symbol	HSD17B11
Gene ID (NCBI)	51170
RRID	AB_2878241
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NBQ5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for HSD17B11 antibody 16303-1-AP	Download protocol
WB protocol for HSD17B11 antibody 16303-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Biosci m6A demethylase FTO promotes tumor progression via regulation of lipid metabolism in esophageal cancer. Authors - Xiaoran Duan View Article

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Validation Data Gallery

WB analysis of L02 using 16303-1-AP

WB analysis of mouse lung using 16303-1-AP

WB analysis of mouse liver using 16303-1-AP