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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using HSCB Recombinant antibody (85185-4-RR)

    WB analysis of rat liver using 85185-4-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85185-4-RR (HSCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85185-4-RR

    Affinity and Kinetic Characterization of 85185-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85185-4-RR against Human HSCB were performed. The affinity constant is below 1 pM.

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HSCB Recombinant antibody

HSCB Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85185-4-RR
Clone No.242836H3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

242836H3, DNAJC20, HSC20, Iron-sulfur cluster co-chaperone protein HscB, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85185-4-RR targets HSCB in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSCB fusion protein Ag7268 相同性解析による交差性が予測される生物種
Full Name HscB iron-sulfur cluster co-chaperone homolog (E. coli)
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC065569
Gene Symbol HSCB
Gene ID (NCBI) 150274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSCB, also named as HSC20 and DNAJC20, acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria.

Protocols

Product Specific Protocols
WB protocol for HSCB antibody 85185-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols