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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using HRH2 Polyclonal antibody (13414-1-AP)

    WB analysis of SH-SY5Y using 13414-1-AP (same clone as 13414-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13414-1-AP (HRH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse stomach tissue using HRH2 Polyclonal antibody (13414-1-AP)

    WB analysis of mouse stomach using 13414-1-AP (same clone as 13414-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13414-1-AP (HRH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using HRH2 Polyclonal antibody (13414-1-AP)

    WB analysis of human skeletal muscle using 13414-1-AP (same clone as 13414-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13414-1-AP (HRH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13414-1-PBS in a different storage buffer formulation.

HRH2 Polyclonal antibody, PBS Only

HRH2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 13414-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13414-1-PBS targets HRH2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4068

Product name: Recombinant human HRH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 290-397 aa of BC054510

Sequence: ALNRDFRTGYQQLFCCRLANRNSHKTSLRSNASQLSRTQSREPRQQEEKPLKLQVWSGTEVTAPQGATDRPWLCLPECWSVELTHSFIHLFIHSFANIHPIPTTCQEL

相同性解析による交差性が予測される生物種
Full Name histamine receptor H2
Calculated molecular weight 397 aa, 45 kDa
Observed molecular weight 59 kDa, 69 kDa
GenBank accession numberBC054510
Gene Symbol HRH2
Gene ID (NCBI) 3274
RRIDAB_10646442
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP25021
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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