Validation Data Gallery
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Tested Applications
| Positive WB detected in | human colon tissue, Jurkat cells |
| Positive IHC detected in | human endometrial cancer tissue, human colon cancer tissue, human trachea tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 7 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
13413-1-AP targets HRH1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4067 Product name: Recombinant human HRH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 211-426 aa of BC060802 Sequence: AKIYKAVRQHCQHRELINRSLPSFSEIKLRPENPKGDAKKPGKESPWEVLKRKPKDAGGGSVLKSPSQTPKEMKSPVVFSQEDDREVDKLYCFPLDIEHMQAAAEGSSRDYVAVNRSHGQLKTDEQGLNTHGASEISEDQMLGDSQSFSRTDSDTTTETAPGKGKLRSGSNTGLDYIKFTWKRLRSHSRQYVSGLHMNRERKAAKQLGFIMAAFIL 相同性解析による交差性が予測される生物種 |
| Full Name | histamine receptor H1 |
| Calculated molecular weight | 487 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC060802 |
| Gene Symbol | HRH1 |
| Gene ID (NCBI) | 3269 |
| RRID | AB_2092454 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35367 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HRH1 antibody 13413-1-AP | Download protocol |
| WB protocol for HRH1 antibody 13413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Reports Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids. | ||
Aging (Albany NY) Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma. | ||
Front Cell Neurosci Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus. | ||
J Virol Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.
| ||
Am J Reprod Immunol Decreased IL-33 Expression in the Cervix in LPS-Induced Preterm Birth and the Potential Role of Mast Cells: A Murine Model |
