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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using HRAS Recombinant antibody (82682-13-RR)

    FC experiment of HeLa using 82682-13-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HRAS-Specific Recombinant Antibody (82682-13-RR, Clone:242062C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Flow cytometry (FC) experiment of HepG2 cells using HRAS Recombinant antibody (82682-13-RR)

    FC experiment of HepG2 using 82682-13-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HRAS-Specific Recombinant Antibody (82682-13-RR, Clone:242062C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

HRAS Recombinant monoclonal antibody

HRAS Uni-rAb® Recombinant Antibody for FC (Intra), ELISA
Cat No. 82682-13-RR
Clone No.242062C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

GTPase HRas, H RASIDX, HRAS1, CTLO, c-H-ras

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82682-13-RR targets HRAS in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
GenBank accession numberBC006499
Gene Symbol HRAS
Gene ID (NCBI) 3265
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP01112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Protocols

Product Specific Protocols
FC protocol for HRAS antibody 82682-13-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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