Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, mouse spleen tissue |
| Positive IHC detected in | human placenta tissue, human heart tissue, human lung tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
13901-1-AP targets HPS5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4867 Product name: Recombinant human HPS5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 661-1016 aa of BC033640 Sequence: CQFLKKYFFLLNLKRAKESIKLSYSNSPSVWDTFIEGLKEMASSNPVYMEMEKGDLPTRLKLLDDEVPFDSPLLVVYATRLYEKFGESALRSLIKFFPSILPSDIIQLCHHHPAEFLAYLDSLVKSRPEDQRSSFLESLLQPESLRLDWLLLAVSLDAPPSTSTMDDEGYPRPHSHLLSWGYSQLILHLIKLPADFITKEKMTDICRSCGFWPGYLILCLELERRREAFTNIVYLNDMSLMKGDNGWIPETVEEWKLLLHLIQSKSTRPAPQESLNGSLSDGPSPINVENVALLLAKAMGPDRAWSLLQECGLALELSEKFTRTCDILRIAEKRQRALIQSMLEKCDRFLWSQQA 相同性解析による交差性が予測される生物種 |
| Full Name | Hermansky-Pudlak syndrome 5 |
| Calculated molecular weight | 1129 aa, 127 kDa |
| Observed molecular weight | 135-141 kDa |
| GenBank accession number | BC033640 |
| Gene Symbol | HPS5 |
| Gene ID (NCBI) | 11234 |
| RRID | AB_2120685 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hermansky-Pudlak syndrome 5 protein (HPS-5), also named Ruby-eye protein 2 homolog (Ru2), plays a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. This protein interacts with Hermansky-Pudlak syndrome 6 protein and may interact with the cytoplasmic domain of integrin, alpha-3. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HPS5 antibody 13901-1-AP | Download protocol |
| IHC protocol for HPS5 antibody 13901-1-AP | Download protocol |
| IF protocol for HPS5 antibody 13901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Am J Hum Genet A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9. | ||
J Invest Dermatol Clinical, molecular, and cellular features of non-Puerto Rican Hermansky-Pudlak syndrome patients of Hispanic descent. | ||
Pigment Cell Melanoma Res A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8. | ||
PLoS One Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5. | ||
Front Genet Oculocutaneous albinism and bleeding diathesis due to a novel deletion in the HPS3 gene |