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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPRT Recombinant antibody (86049-1-RR)

    WB analysis using 86049-1-RR (same clone as 86049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86049-1-RR (HPRT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human colon cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human colon cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human breast cancer using 86049-1-RR (same clone as 86049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86049-1-PBS in a different storage buffer formulation.

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HPRT Recombinant antibody, PBS Only

HPRT Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86049-1-PBS
Clone No.250656C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86049-1-PBS targets HPRT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HPRT fusion protein Ag7044 相同性解析による交差性が予測される生物種
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated molecular weight 25 kDa
Observed molecular weight 24-28 kDa
GenBank accession numberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00492
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)