• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HPDL Polyclonal antibody

HPDL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20777-1-AP

Synonyms

4 HPPD L, GLOXD1, HPDL, RP4 534D1.1

Validation Data Gallery

Applications:
  • WB analysis using 20777-1-AP

    WB analysis using 20777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 20777-1-AP

    WB analysis of L02 using 20777-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 20777-1-AP

    WB analysis of L02 using 20777-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20777-1-AP (HPDL antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20777-1-AP

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IHC staining of human stomach using 20777-1-AP

    IHC staining of human stomach using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IHC staining of human colon using 20777-1-AP

    IHC staining of human colon using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IHC staining of human colon using 20777-1-AP

    IHC staining of human colon using 20777-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20777-1-AP (HPDL antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HPDL antibody included
  • IF Staining of Caco-2 using 20777-1-AP

    IF Staining of Caco-2 using 20777-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using HPDL antibody (20777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy HPDL Ready-To-Use IHC Kit. HPDL primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20777-1-AP targets HPDL in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPDL fusion protein Ag14773 相同性解析による交差性が予測される生物種
Full Name 4-hydroxyphenylpyruvate dioxygenase-like
Calculated molecular weight 371 aa, 39 kDa
Observed molecular weight 35-39 kDa
GenBank accession numberBC007293
Gene symbol HPDL
Gene ID (NCBI) 84842
RRIDAB_10697807
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HPDL antibody 20777-1-APDownload protocol
IHC protocol for HPDL antibody 20777-1-APDownload protocol
IF protocol for HPDL antibody 20777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols