Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10989-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10989-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10989-1-AP (VILIP 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 10989-1-AP
IP result of anti-VILIP3 (IP:10989-1-AP, 4ug; Detection:10989-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.
IHC staining of human kidney using 10989-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 10989-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 10989-1-AP (VILIP 3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse cerebellum using 10989-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 10989-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10989-1-AP (VILIP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10989-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VILIP3 antibody (10989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

VILIP3 Polyclonal antibody

VILIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10989-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

HPCAL1, Calcium-binding protein BDR-1, hippocalcin like 1, VILIP-3, Visinin-like protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, HeLa cells, mouse brain tissue, rat brain tissue, human brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human kidney tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 4 publications below
IF	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10989-1-AP targets VILIP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1449 Product name: Recombinant human HPCA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC009846 Sequence: MGKQNSKLRPEVLQDLRENTEFTDHELQEWYKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRSEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF 相同性解析による交差性が予測される生物種
Full Name	hippocalcin-like 1
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC009846
Gene Symbol	VILIP3
Gene ID (NCBI)	3241
RRID	AB_2120220
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P37235
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VILIP3, is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system.

Protocols

Product Specific Protocols
IF protocol for VILIP3 antibody 10989-1-AP	Download protocol
IHC protocol for VILIP3 antibody 10989-1-AP	Download protocol
IP protocol for VILIP3 antibody 10989-1-AP	Download protocol
WB protocol for VILIP3 antibody 10989-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis Authors - Xin Chen KD Validated View Article
mouse	IF	Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. Authors - I-Ling Lu View Article
human	WB,IHC	Hepatology Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization via activating ERK1/2-MAPK pathway. Authors - Yonglong Zhang KD Validated View Article
human	WB,IHC	Cancer Med Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments. Authors - Mingjian Ma View Article
mouse,human	WB	Theranostics Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling Authors - Tao Chen View Article
human	IHC	Signal Transduct Target Ther Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression Authors - Xiang-Peng Tan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10989-1-AP

WB analysis of HeLa using 10989-1-AP

IP experiment of mouse brain using 10989-1-AP

IHC staining of human kidney using 10989-1-AP

IHC staining of human kidney using 10989-1-AP

IHC staining of mouse cerebellum using 10989-1-AP

IHC staining of mouse cerebellum using 10989-1-AP

IF Staining of HeLa using 10989-1-AP

VILIP3 Polyclonal antibody

VILIP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of HPCAL1 as a specific autophagy receptor involved in ferroptosis

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization via activating ERK1/2-MAPK pathway.

Expressional and prognostic value of HPCAL1 in cholangiocarcinoma via integrated bioinformatics analyses and experiments.

Hippocalcin-Like 1 blunts liver lipid metabolism to suppress tumorigenesis via directly targeting RUVBL1-mTOR signaling

Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression