Validation Data Gallery

Filter: AllWB

WB analysis of A375 using 18588-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 18588-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18588-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 18588-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 18588-1-AP (HOXD8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

HOXD8 Polyclonal antibody

HOXD8 Polyclonal Antibody for WB, ELISA

Cat No. 18588-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Homeobox protein Hox-4E, Homeobox protein Hox-5.4, Homeobox protein Hox-D8, HOX4E

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

A375 cells, HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

18588-1-AP targets HOXD8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13245 Product name: Recombinant human HOXD8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-289 aa of BC038709 Sequence: MSSYFVNPLYSKYKAAAAAAAAAGEAINPTYYDCHFAPEVGGRHAAAAAALQLYGNSAAGFPHAPPQAHAHPHPSPPPSGTGCGGREGRGQEYFHPGGGSPAAAYQAAPPPPPHPPPPPPPPPCGGIACHGEPAKFYGYDNLQRQPIFTTQQEAELVQYPDCKSSSGNIGEDPDHLNQSSSPSQMFPWMRPQAPGRRRGRQTYSRFQTLELEKEFLFNPYLTRKRRIEVSHALALTERQVKIWFQNRRMKWKKENNKDKFPVSRQEVKDGETKKEAQELEEDRAEGLTN 相同性解析による交差性が予測される生物種
Full Name	homeobox D8
Calculated molecular weight	32 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC038709
Gene Symbol	HOXD8
Gene ID (NCBI)	3234
RRID	AB_2878554
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13378
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HOXD8 antibody 18588-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Exp Cell Res HOXD8 Drives Glioma Progression through Epithelial-Mesenchymal Transition Regulation: Implications for Prognosis and Targeted Therapy Authors - Ke Yu View Article

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