Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of A549 using 18736-1-AP (same clone as 18736-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
WB analysis of mouse lung using 18736-1-AP (same clone as 18736-1-PBS)
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 18736-1-AP (same clone as 18736-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 18736-1-AP (same clone as 18736-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 18736-1-AP (same clone as 18736-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 18736-1-AP (HOXD13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IP experiment of A549 using 18736-1-AP (same clone as 18736-1-PBS)
IP result of anti-HOXD13 (IP:18736-1-AP, 4ug; Detection:18736-1-AP 1:500) with A549 cells lysate 1600ug. This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18736-1-AP (same clone as 18736-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18736-1-AP (HOXD13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18736-1-PBS in a different storage buffer formulation.

HOXD13 Polyclonal antibody, PBS Only

HOXD13 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 18736-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18736-1-PBS targets HOXD13 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	homeobox D13
Calculated molecular weight	343 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC148863
Gene Symbol	HOXD13
Gene ID (NCBI)	3239
RRID	AB_2119926
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35453
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

HOXD13, also named as HOX4I, belongs to the Abd-B homeobox family. HOXD13 is a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Mutation of HOXD13 will cause synpolydactyly (SPD), brachydactyly type D (BDD), syndactyly type 5brachydactyly-syndactyly syndrome (BDSD) or brachydactyly type E (BDE).Present polyclonal anti-HOX13D antibody(18736-1-AP) is produced by immunizing animals with part of N-terminus chain of HOX13D and detect a 36-kDa band in cell and tissues.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 18736-1-AP (same clone as 18736-1-PBS)

WB analysis of mouse lung using 18736-1-AP (same clone as 18736-1-PBS)

WB analysis of mouse brain using 18736-1-AP (same clone as 18736-1-PBS)

WB analysis of Jurkat using 18736-1-AP (same clone as 18736-1-PBS)

WB analysis of rat brain using 18736-1-AP (same clone as 18736-1-PBS)

IP experiment of A549 using 18736-1-AP (same clone as 18736-1-PBS)

IHC staining of human brain using 18736-1-AP (same clone as 18736-1-PBS)