Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, PC-12 cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
14321-1-AP targets HOXC4 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HOXC4 fusion protein Ag5563 相同性解析による交差性が予測される生物種 |
| Full Name | homeobox C4 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC050442 |
| Gene Symbol | HOXC4 |
| Gene ID (NCBI) | 3221 |
| RRID | AB_2233196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09017 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The class I homeobox (HOX) family of homeodomain-containing transcription factors has 39 members in mammals. These genes are distributed throughout the genome in four clusters: HOXA (7p15), HOXB (17q21), HOXC (12q13), and HOXD (2q31). HOX genes are known to act as transcription factors, playing an essential regulatory role in the temporal and spatial development of the embryo. HOXC family members are not expressed in normal prostate tissue but increased expression of HOXC genes is commonly detected in prostate cancers. HOXC4 is expressed in GC B cells and HoxC4 expression is specifically up-regulated by the same stimuli that induce AID expression (LPS, CD154 and IL-4). The calculated molecular weight of HOXC4 is 30 kDa. Sometimes higher molecular weight around 39 kDa can also be observed, which may be a modified variant of HOXC4 (PMID: 23178148, 32012197, 35400027).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HOXC4 antibody 14321-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncotarget Long noncoding RNA uc.345 promotes tumorigenesis of pancreatic cancer by upregulation of hnRNPL expression. |