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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    WB analysis of HeLa using 20632-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    WB analysis of HeLa using 20632-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HOXC10 Polyclonal antibody (20632-1-AP)

    IF Staining of HeLa using 20632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20632-1-AP (HOXC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXC10 Polyclonal antibody

HOXC10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20632-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20632-1-AP targets HOXC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14620

Product name: Recombinant human HOXC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC001293

Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTG

相同性解析による交差性が予測される生物種
Full Name homeobox C10
Calculated molecular weight 342 aa, 38 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001293
Gene Symbol HOXC10
Gene ID (NCBI) 3226
RRIDAB_10697666
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)

Protocols

Product Specific Protocols
WB protocol for HOXC10 antibody 20632-1-APDownload protocol
IF protocol for HOXC10 antibody 20632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols