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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using HOXB9 Recombinant antibody (84609-3-RR)

    WB analysis of K-562 using 84609-3-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HOXB9 Recombinant antibody (84609-3-RR)

    WB analysis using 84609-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84609-3-RR (HOXB9 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84609-3-RR

    Affinity and Kinetic Characterization of 84609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84609-3-RR against Human HOXB9 were performed. The affinity constant is 40.5 nM.

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HOXB9 Recombinant antibody

HOXB9 Recombinant Antibody for WB, ELISA
Cat No. 84609-3-RR
Clone No.241866C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HOX2E, HOX2.5, Homeobox protein Hox-B9, Homeobox protein Hox-2E, Homeobox protein Hox-2.5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, HCT 116 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84609-3-RR targets HOXB9 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HOXB9 fusion protein Ag34837 相同性解析による交差性が予測される生物種
Full Name homeobox B9
Calculated molecular weight 250 aa, 28 kDa
Observed molecular weight30 kDa
GenBank accession numberBC015565
Gene Symbol HOXB9
Gene ID (NCBI) 3219
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17482
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXB9 is a member of the Homeobox (HOX) gene family, which plays a crucial role in embryonic development and is involved in the regulation of gene expression during this process. In addition to its role in embryogenesis, HOXB9 has been implicated in various cancers, including breast and prostate cancer. It is overexpressed in breast cancer tissue and is regulated by estrogen, playing a critical role in angiogenesis. HOXB9 overexpression has been shown to stimulate three-dimensional colony formation in soft-agar assays, indicating its role in tumorigenesis. Furthermore, HOXB9 binds to the promoters of various tumor growth and angiogenic factors, regulating their expression, and its homeodomain plays a crucial role in transcriptional regulation and tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for HOXB9 antibody 84609-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols