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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis using 21653-1-AP (same clone as 21653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis using 21653-1-AP (same clone as 21653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis of mouse brain using 21653-1-AP (same clone as 21653-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21653-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HOXB1 Polyclonal antibody (21653-1-AP)

    WB analysis of NIH/3T3 using 21653-1-AP (same clone as 21653-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21653-1-AP (HOXB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21653-1-PBS in a different storage buffer formulation.

HOXB1 Polyclonal antibody, PBS Only

HOXB1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 21653-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

homeobox B1, Homeobox protein Hox 2I, Homeobox protein Hox B1, Homeobox protein Hox-2I, Homeobox protein Hox-B1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21653-1-PBS targets HOXB1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16366

Product name: Recombinant human HOXB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-168 aa of BC096192

Sequence: MDYNRMNSFLEYPLCNRGPSAYSAHSAHSAPTSFPPSSAQAVDSYASEGRYGGGLSSPAFQQNSGYPAQQPPSTLGVPFPSSAPSGYAPAACSPSYGPSQYYPLGHSEGDGGYFHPSSYGAQLGGLSDGYGAGGAGPGPYPPQHPPYGNEQTASFAPAYADLLSEDKE

相同性解析による交差性が予測される生物種
Full Name homeobox B1
Calculated molecular weight 235 aa, 26 kDa
Observed molecular weight32, 25 kDa
GenBank accession numberBC096192
Gene Symbol HOXB1
Gene ID (NCBI) 3211
RRIDAB_2878899
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP14653
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HOXB1, also named as HOX2I, belongs to the Antp homeobox family and the Labial subfamily. HOXB1 is sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXB1 acts on the anterior body structures. Establishment of the spatial colinearity in the embryo is directly controlled by the Hox genes themselves. 21653-1-AP is a rabbit polyclonal antibody raised against residues near the N-terminus of human HOXB1.

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