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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293 cells using HOXA9 Polyclonal antibody (29978-1-AP)

    IP experiment of HEK-293 using 29978-1-AP

    IP result of anti-HOXA9 (IP:29978-1-AP, 4ug; Detection:29978-1-AP 1:800) with HEK-293 cells lysate 1480 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using HOXA9 Polyclonal antibody (29978-1-AP)

    IHC staining of human colon using 29978-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29978-1-AP (HOXA9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HOXA9 Polyclonal antibody

HOXA9 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 29978-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, ELISA

Synonyms

HOX1G, HOX1.7, HOX1, Homeobox protein Hox-A9, Homeobox protein Hox-1G

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29978-1-AP targets HOXA9 in IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32309

Product name: Recombinant human HOXA9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-199 aa of BC010023

Sequence: VYHHHHHHPYVHPQAPVAAAAPDGRYMRSWLEPTPGALSFAGLPSSRPYGIKPEPLSARRGDCPTLDTHTLSLTDYACGSPPVDREKQPSEGAFSENNAENESGGDKPPIDPNNPAAN

相同性解析による交差性が予測される生物種
Full Name homeobox A9
Calculated molecular weight 30 kDa
Observed molecular weight36 kDa
GenBank accession numberBC010023
Gene Symbol HOXA9
Gene ID (NCBI) 3205
RRIDAB_3086202
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31269
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homeobox protein Hox-A9 (HOXA9) also known as HOX1G,a member of HOX family belonging to the HOXA cluster, is often studied in acute myeloid leukemia (AML), which is linked to proliferation, differentiation, and progenitor self-renewal maintenance. It is located in nucleoplasm and functions as a critical regulator of hematopoiesis, essential for the maintenance of hematopoietic stem cells and their differentiation into myeloid lineages (PMID: 34743404). Many post-transcriptional events including microRNAs, long non-coding RNAs, and epigenetic modification could also determine HOXA9 protein level and function (PMID: 36376832).

Protocols

Product Specific Protocols
IHC protocol for HOXA9 antibody 29978-1-APDownload protocol
IP protocol for HOXA9 antibody 29978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols