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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis using 67112-1-Ig (same clone as 67112-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis using 67112-1-Ig (same clone as 67112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of THP-1 using 67112-1-Ig (same clone as 67112-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HepG2 using 67112-1-Ig (same clone as 67112-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of SMMC-7721 using 67112-1-Ig (same clone as 67112-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HT-29 using 67112-1-Ig (same clone as 67112-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of HL-60 using 67112-1-Ig (same clone as 67112-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    WB analysis of K-562 using 67112-1-Ig (same clone as 67112-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67112-1-Ig (HOXA7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HOXA7 Monoclonal antibody (67112-1-Ig)

    IF Staining of HepG2 using 67112-1-Ig (same clone as 67112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67112-1-Ig (HOXA7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67112-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HOXA7 Monoclonal antibody, PBS Only

HOXA7 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67112-1-PBS
Clone No.2A7H6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

2A7H6, ANTP, Homeobox protein Hox-1A, Homeobox protein Hox-A7, HOX1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67112-1-PBS targets HOXA7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19025

Product name: Recombinant human HOXA7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-110 aa of BC148692

Sequence: MSSSYYVNALFSKYTAGASLFQNAEPTSCSFAPNSQRSGYGAGAGAFASTVPGLYNVNSPLYQSPFASGYGLGADAYGNLPCASYDQNIPGLCSDLAKGACDKTDEGALH

相同性解析による交差性が予測される生物種
Full Name homeobox A7
Calculated molecular weight 230 aa, 25 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC148692
Gene Symbol HOXA7
Gene ID (NCBI) 3204
RRIDAB_2882416
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP31268
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta.

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