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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOPX Polyclonal antibody (11419-1-AP)

    WB analysis using 11419-1-AP (same clone as 11419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using HOPX Polyclonal antibody (11419-1-AP)

    WB analysis of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11419-1-AP (HOPX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HOPX Polyclonal antibody (11419-1-AP)

    IHC staining of human placenta using 11419-1-AP (same clone as 11419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HOPX Polyclonal antibody (11419-1-AP)

    IHC staining of human placenta using 11419-1-AP (same clone as 11419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using HOPX Polyclonal antibody (11419-1-AP)

    IHC staining of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using HOPX Polyclonal antibody (11419-1-AP)

    IHC staining of mouse lung using 11419-1-AP (same clone as 11419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11419-1-AP (HOPX antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11419-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HOPX Polyclonal antibody, PBS Only

HOPX Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11419-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11419-1-PBS targets HOPX in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1979

Product name: Recombinant human HOPX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-73 aa of BC014225

Sequence: MSAETASGPTEDQVEILEYNFNKVDKHPDSTTLCLIAAEAGLSEEETQKWFKQRLAKWRRSEGLPSECRSVID

相同性解析による交差性が予測される生物種
Full Name HOP homeobox
Calculated molecular weight 73 aa, 8 kDa
Observed molecular weight 8-12 kDa
GenBank accession numberBC014225
Gene Symbol HOPX
Gene ID (NCBI) 84525
RRIDAB_10693525
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPY8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HOPX (Homeodomain-only protein) gene has various synonyms including HOD, HOP, OB1, LAGY and NECC1. The protein encoded by this gene is an unusual homeodomain protein that lacks certain conserved residues required for DNA binding. HOPX has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. HOPX protein acts as an antagonist to the serum response factor (SRF), which regulates the opposing processes of cell proliferation and differentiation. Overexpression of HOPX causes cardiac hypertrophy. HOPX protein can inhibit SRF-dependent transcriptional activation by recruiting histone deacetylase (HDAC) activity.

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