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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOOK2 Polyclonal antibody (32110-1-AP)

    WB analysis using 32110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32110-1-AP (HOOK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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HOOK2 Polyclonal antibody

HOOK2 Polyclonal Antibody for WB, ELISA
Cat No. 32110-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Hook Microtubule Tethering Protein 2, hook homolog 2 (Drosophila), HK2, h-hook2, hHK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32110-1-AP targets HOOK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOOK2 fusion protein Ag37279 相同性解析による交差性が予測される生物種
Full Name hook homolog 2 (Drosophila)
Calculated molecular weight83 kDa
Observed molecular weight80 kDa
GenBank accession numberNM_013312
Gene Symbol HOOK2
Gene ID (NCBI) 29911
RRIDAB_3670194
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96ED9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOOK2 (hook microtubule-tethering protein 2) is a microtubule-binding protein belonging to the Hook family of proteins.Hook2 normally functions by attaching microtubules to subcellular structures and is involved in a variety of cellular processes, including endosomal/lysosomal processing, Golgi and centrosomal organization, and functional maintenance.Hook2 is Hook2 is required for the assembly of the dynamin-dynamic actin complex and also plays an important role in regulating PAR6α recruitment to centrosomes to bridge microtubules and the aPKC/PAR complex. Therefore, it may be relevant to cell migration, organelle organization, and signaling-related diseases.

Protocols

Product Specific Protocols
WB protocol for HOOK2 antibody 32110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols