Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32110-1-AP targets HOOK2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37279 Product name: Recombinant human HOOK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 180-400 aa of NM_013312 Sequence: LSEEAEEGDELQQRCLDLERQLMLLSEEKQSLAQENAGLRERMGRPEGEGTPGLTAKKLLLLQSQLEQLQEENFRLESGREDERLRCAELEREVAELQHRNQALTSLAQEAQALKDEMDELRQSSERAGQLEATLTSCRRRLGELRELRRQVRQLEERNAGHAERTRQLEDELRRAGSLRAQLEAQRRQVQELQGQRQEEAMKAEKWLFECRNLEEKYESV 相同性解析による交差性が予測される生物種 |
| Full Name | hook homolog 2 (Drosophila) |
| Calculated molecular weight | 83 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | NM_013312 |
| Gene Symbol | HOOK2 |
| Gene ID (NCBI) | 29911 |
| RRID | AB_3670194 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96ED9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HOOK2 (hook microtubule-tethering protein 2) is a microtubule-binding protein belonging to the Hook family of proteins.Hook2 normally functions by attaching microtubules to subcellular structures and is involved in a variety of cellular processes, including endosomal/lysosomal processing, Golgi and centrosomal organization, and functional maintenance.Hook2 is Hook2 is required for the assembly of the dynamin-dynamic actin complex and also plays an important role in regulating PAR6α recruitment to centrosomes to bridge microtubules and the aPKC/PAR complex. Therefore, it may be relevant to cell migration, organelle organization, and signaling-related diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HOOK2 antibody 32110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |