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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HOOK2 Polyclonal antibody

HOOK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12458-1-AP

Synonyms

h hook2, hHK2, HK2, hook homolog 2 (Drosophila), HOOK2, Protein Hook homolog 2

Validation Data Gallery

Applications:
  • WB analysis using 12458-1-AP

    WB analysis using 12458-1-AP

    SH-SY5Y cell lysates were subjected to SDS PAGE followed by western blot with 12458-1-AP (HOOK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 12458-1-AP

    IHC staining of mouse colon using 12458-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12458-1-AP (HOOK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12458-1-AP targets HOOK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOOK2 fusion protein Ag3124 相同性解析による交差性が予測される生物種
Full Name hook homolog 2 (Drosophila)
Calculated molecular weight 717 aa, 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC012443
Gene symbol HOOK2
Gene ID (NCBI) 29911
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HOOK2 antibody 12458-1-APDownload protocol
IHC protocol for HOOK2 antibody 12458-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols