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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HOOK1 Polyclonal antibody (10871-1-AP)

    WB analysis of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using HOOK1 Polyclonal antibody (10871-1-AP)

    WB analysis of human brain using 10871-1-AP (same clone as 10871-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HOOK1 Polyclonal antibody (10871-1-AP)

    IP experiment of mouse brain using 10871-1-AP (same clone as 10871-1-PBS)

    IP result of anti-HOOK1 (IP:10871-1-AP, 4ug; Detection:10871-1-AP 1:500) with mouse brain tissue lysate 4680ug. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HOOK1 Polyclonal antibody (10871-1-AP)

    IHC staining of human colon cancer using 10871-1-AP (same clone as 10871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10871-1-AP (HOOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using HOOK1 Polyclonal antibody (10871-1-AP)

    IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 10871-1-AP (HOOK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using HOOK1 Polyclonal antibody (10871-1-AP)

    IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HOOK1 antibody (10871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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HOOK1 Polyclonal antibody, PBS Only

HOOK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10871-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Protein Hook homolog 1, hook homolog 1 (Drosophila), HK1, h-hook1, hHK1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10871-1-PBS targets HOOK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOOK1 fusion protein Ag1311 相同性解析による交差性が予測される生物種
Full Name hook homolog 1 (Drosophila)
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC011621
Gene Symbol HOOK1
Gene ID (NCBI) 51361
RRIDAB_2120812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJC3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.