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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOMER1 Recombinant antibody (85692-4-RR)

    WB analysis using 85692-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HOMER1 Recombinant antibody (85692-4-RR)

    WB analysis using 85692-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85692-4-RR (HOMER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Recombinant antibody (85692-4-RR)

    IHC staining of mouse brain using 85692-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HOMER1 Recombinant antibody (85692-4-RR)

    IHC staining of mouse brain using 85692-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85692-4-RR (HOMER1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MOLT-4 cells using HOMER1 Recombinant monoclonal antibody (85692-4-RR)

    IF Staining of MOLT-4 using 85692-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using HOMER1 antibody (85692-4-RR, Clone: 243007G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85692-4-RR

    Affinity and Kinetic Characterization of 85692-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85692-4-RR against Human HOMER1 were performed. The affinity constant is 0.309 nM.

HOMER1 Recombinant monoclonal antibody

HOMER1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85692-4-RR
Clone No.243007G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HOMER, Homer 1, homer homolog 1 (Drosophila), Homer protein homolog 1, Homer-1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, MOLT-4 cells, HEK-293 cells, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85692-4-RR targets HOMER1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3109

Product name: Recombinant human HOMER1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-354 aa of BC015502

Sequence: FSTRAHVFQIDPNTKKNWVPTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMTFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFAEKFQEFKEAARLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVTELECVSSQANAVHTHKTELNQTIQELEETLKLKEEEIERLKQEIDNARELQEQRDSLTQKLQEVEIRNKDLEGQLSDLEQRLEKSQNEQEAFRNNLKTLLEILDGKIFELTELRDNLAKLLECS

相同性解析による交差性が予測される生物種
Full Name homer homolog 1 (Drosophila)
Calculated molecular weight 40 kDa
Observed molecular weight 46-48 kDa
GenBank accession numberBC015502
Gene Symbol HOMER1
Gene ID (NCBI) 9456
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ86YM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human HOMER1, also named as SYN47, belongs to the Homer family. Human HOMER1 has some isoforms: HOMER1a, HOMER1b, HOMER1c, HOMER1e and HOMER1h. Mouse Homer1 also named as Vesl-1 and it has some isoforms: Vesl-1l, Vesl-1m, Vesl-1s, Homer1b, and Homer1d. HOMER1 is a postsynaptic density scaffolding protein. It is a neuronal immediate-early gene (IEG) that is enriched at excitatory synapses and binds mGluRs. In WB, the MW of HOMER1 is about 46-50kd, 40-43kd and 20-26kd.

Protocols

Product Specific Protocols
IHC protocol for HOMER1 antibody 85692-4-RRDownload protocol
WB protocol for HOMER1 antibody 85692-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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