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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRPDL Recombinant antibody (83129-5-RR)

    WB analysis using 83129-5-RR (same clone as 83129-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83129-5-RR (HNRPDL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83129-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using HNRPDL Recombinant antibody (83129-5-RR)

    IF Staining of U-251 using 83129-5-RR (same clone as 83129-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using HNRPDL antibody (83129-5-RR, Clone: 230415F9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83129-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HNRPDL Recombinant antibody (83129-5-RR)

    FC experiment of HeLa using 83129-5-RR (same clone as 83129-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HNRPDL Recombinant antibody (83129-5-RR, Clone:230415F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83129-5-PBS in a different storage buffer formulation.

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HNRPDL Recombinant antibody, PBS Only

HNRPDL Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83129-5-PBS
Clone No.230415F9

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

hnRNP DL, hnRNP D like, HNRNP, Heterogeneous nuclear ribonucleoprotein D-like, AU-rich element RNA-binding factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83129-5-PBS targets HNRPDL in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRPDL fusion protein Ag1046 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D-like
Calculated molecular weight 46 aa, 4 kDa
GenBank accession numberBC007392
Gene Symbol HNRPDL
Gene ID (NCBI) 9987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14979
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.