Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86926-3-RR targets HNRNPUL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0899 Product name: Recombinant human HNRNPUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC002564 Sequence: MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGSELEGTAQPPPPGLQPHAEPGCYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPEMKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTLVAIDTYNCDLHFKVARD 相同性解析による交差性が予測される生物種 |
| Full Name | heterogeneous nuclear ribonucleoprotein U-like 1 |
| Calculated molecular weight | 120 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC002564 |
| Gene Symbol | HNRNPUL1 |
| Gene ID (NCBI) | 11100 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BUJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HNRNPUL1, also named as E1BAP5, HNRPUL1 and E1B-AP5, acts as a basic transcriptional regulator. It represses basic transcription driven by several virus and cellular promoters. HNRNPUL1 plays also a role in mRNA processing and transport. It is a cellular protein that interacts with the adenovirus protein E1B-55 and implicated in mRNA export. HNRNPUL1 is an hnRNP-like protein that likely mediates the interaction of NXF1 with mRNAs.(PMID:17267598). It may modulate PAN expression in complex with KSHV ORF57(PMID:22043172). HNRNPUL1 specifically bound to the Rae1-Nup98 complex independently of M protein, and this interaction was found to be RNA dependent, as it was greatly diminished in the presence of RNase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNRNPUL1 antibody 86926-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |