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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPU Recombinant monoclonal antibody (84964-4-RR)

    WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPU Recombinant monoclonal antibody (84964-4-RR)

    IHC staining of mouse colon using 84964-4-RR (same clone as 84964-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using HNRNPU Recombinant monoclonal antibody (84964-4-RR)

    IHC staining of mouse colon using 84964-4-RR (same clone as 84964-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPU Recombinant monoclonal antibody (84964-4-RR)

    IF Staining of HeLa using 84964-4-RR (same clone as 84964-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (84964-4-RR, Clone: 242609C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using HNRNPU Recombinant monoclonal antibody (84964-4-RR)

    FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

HNRNPU Recombinant monoclonal antibody, PBS Only

HNRNPU Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84964-4-PBS
Clone No.242609C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242609C9, C1orf199, GRIP120, hnRNP U, HNRPU

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84964-4-PBS targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6141

Product name: Recombinant human HNRNPU protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 10-236 aa of BC003621

Sequence: KLKVSELKEELKKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRSGAGLEQEAAAGGDEEEEEEEEEEEGISALDGDQMELGEENGAAGAADSGPMEEEEAASEDENGDDQGFQEGEDELGDEEEGAGDENGHGEQQPQPPATQQQQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTEQKGGDKKRGVKRPREDHGR

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight 825 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC003621
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
RRIDAB_3743891
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ00839
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

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