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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPU Recombinant antibody (84964-4-RR)

    WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using HNRNPU Recombinant antibody (84964-4-RR)

    FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

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HNRNPU Recombinant antibody, PBS Only

HNRNPU Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84964-4-PBS
Clone No.242609C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Nuclear p120 ribonucleoprotein, HNRPU, hnRNP U, GRIP120, C1orf199

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84964-4-PBS targets HNRNPU in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HNRNPU fusion protein Ag6141 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight 825 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC003621
Gene Symbol HNRNPU
Gene ID (NCBI) 3192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00839
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).