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KD/KO Validated

HNRNPU Polyclonal antibody

Name: HNRNPU Polyclonal antibody
Brand: Proteintech
SKU: 16365-1-AP

HNRNPU Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16365-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1

Validation Data Gallery

Applications:

AllWBIPIHCIFFC

WB analysis of HEK-293 using 16365-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16365-1-AP
WB result of HNRNPU antibody (16365-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HNRNPU transfected HeLa cells.
WB analysis using 16365-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16365-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16365-1-AP (HNRNPU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16365-1-AP
IP result of anti-HNRNPU (IP:16365-1-AP, 4ug; Detection:16365-1-AP 1:8000) with HeLa cells lysate 2000 ug.
IHC staining of mouse kidney using 16365-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HNRNPU antibody included
IHC staining of mouse kidney using 16365-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16365-1-AP (HNRNPU antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with HNRNPU antibody included
IF Staining of HeLa using 16365-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16365-1-AP (HNRNPU antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 16365-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 16365-1-AP (HNRNPU antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 16365-1-AP
1X10^6 HepG2 cells were stained with 0.2ug HNRNPU antibody (16365-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Cat No. 16365-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, mouse testis tissue
Positive FC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

16365-1-AP targets HNRNPU in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HNRNPU fusion protein Ag9151 相同性解析による交差性が予測される生物種
Full Name	heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight	91 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC003367
Gene symbol	HNRNPU
Gene ID (NCBI)	3192
RRID	AB_10596481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 16365-1-AP	Download protocol
IHC protocol for HNRNPU antibody 16365-1-AP	Download protocol
IF protocol for HNRNPU antibody 16365-1-AP	Download protocol
IP protocol for HNRNPU antibody 16365-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia. Authors - Yanni Ma View Article
human	IF	Cancer Res DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing. Authors - Songge Xing View Article
human	IP,IF	mSystems Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia. Authors - Xiaoqing Liu View Article
		Cell Prolif Protein phosphatase 4 plays dual roles during cell proliferation. Authors - Xiuqing Huang View Article
human	WB	J Exp Clin Cancer Res A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. Authors - Xiaozhu Tang KD Validated View Article
human	IHC,WB	Front Oncol The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma. Authors - Yixin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 16365-1-AP

WB analysis of HeLa using 16365-1-AP

WB analysis using 16365-1-AP

WB analysis of HeLa using 16365-1-AP

IP experiment of HeLa using 16365-1-AP

IHC staining of mouse kidney using 16365-1-AP

IHC staining of mouse kidney using 16365-1-AP

IF Staining of HeLa using 16365-1-AP

IF Staining of mouse testis using 16365-1-AP

FC experiment of HepG2 using 16365-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Protein phosphatase 4 plays dual roles during cell proliferation.

A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing.

The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.