Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, MCF-7 cells, HeLa cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human tonsillitis tissue, human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
26897-1-AP targets HNRNPM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25474 Product name: Recombinant human HNRNPM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-93 aa of BC000138 Sequence: MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKE 相同性解析による交差性が予測される生物種 |
| Full Name | heterogeneous nuclear ribonucleoprotein M |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 73-77 kDa |
| GenBank accession number | BC000138 |
| Gene Symbol | HNRNPM |
| Gene ID (NCBI) | 4670 |
| RRID | AB_2880673 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52272 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HNRNPM, also named as NAGR1, is a 730 amino acid protein, which localizes in nucleus. HNRNPM as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. HNRNPM is Involved in splicing. HNRNPM acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines. HNRNPM exists two isoforms with MV 77 kDa and 73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPM antibody 26897-1-AP | Download protocol |
| IHC protocol for HNRNPM antibody 26897-1-AP | Download protocol |
| IP protocol for HNRNPM antibody 26897-1-AP | Download protocol |
| WB protocol for HNRNPM antibody 26897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Nat Commun Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.
| ||
Proc Natl Acad Sci U S A CircURI1 interacts with hnRNPM to inhibit metastasis by modulating alternative splicing in gastric cancer. | ||
J Exp Clin Cancer Res PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α. | ||
Nucleic Acids Res Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to interferon induction | ||
Sci Rep Unraveling the independent role of METTL3 in m6A modification and tumor progression in esophageal squamous cell carcinoma |