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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPH3 Polyclonal antibody (17674-1-AP)

    WB analysis using 17674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17674-1-AP (HNRNPH3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using HNRNPH3 Polyclonal antibody (17674-1-AP)

    IF Staining of PC-3 using 17674-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HNRNPH3 antibody (17674-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using HNRNPH3 Polyclonal antibody (17674-1-AP)

    IF Staining of PC-3 using 17674-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HNRNPH3 antibody (17674-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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HNRNPH3 Polyclonal antibody

HNRNPH3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17674-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HNRPH3, hnRNP H3, hnRNP 2H9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, mouse testis tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17674-1-AP targets HNRNPH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPH3 fusion protein Ag11611 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein H3 (2H9)
Calculated molecular weight 331 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC039824
Gene Symbol HNRNPH3
Gene ID (NCBI) 3189
RRIDAB_2878425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HNRNPH3 antibody 17674-1-APDownload protocol
IF protocol for HNRNPH3 antibody 17674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Dis Model Mech

Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.

Authors - Kyoung-In Cho
View Article