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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPH1 Monoclonal antibody (67375-1-Ig)

    WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HNRNPH1 Monoclonal antibody (67375-1-Ig)

    WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HNRNPH1 Monoclonal antibody (67375-1-Ig)

    IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HNRNPH1 Monoclonal antibody (67375-1-Ig)

    IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPH1 Monoclonal antibody (67375-1-Ig)

    IF Staining of HepG2 using 67375-1-Ig (same clone as 67375-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

HNRNPH1 Monoclonal antibody, PBS Only

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67375-1-PBS
Clone No.1A6C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67375-1-PBS targets HNRNPH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6603

Product name: Recombinant human HNRNPH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 104-449 aa of BC001348

Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein H1 (H)
Calculated molecular weight 49 kDa
Observed molecular weight 49-50 kDa
GenBank accession numberBC001348
Gene Symbol HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2882623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP31943
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

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