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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPD Monoclonal antibody (68236-1-Ig)

    WB analysis using 68236-1-Ig (same clone as 68236-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68236-1-Ig (HNRNPD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HNRNPD Monoclonal antibody (68236-1-Ig)

    IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HNRNPD Monoclonal antibody (68236-1-Ig)

    IHC staining of human cervical cancer using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68236-1-Ig (HNRNPD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRNPD Monoclonal antibody (68236-1-Ig)

    IF Staining of HepG2 using 68236-1-Ig (same clone as 68236-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPD antibody (68236-1-Ig, Clone: 2G9E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68236-1-PBS in a different storage buffer formulation.

HNRNPD Monoclonal antibody, PBS Only

HNRNPD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68236-1-PBS
Clone No.2G9E6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68236-1-PBS targets HNRNPD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3458

Product name: Recombinant human HNRNPD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC026015

Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGSAESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKFGEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEPVKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIMEKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQGYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa)
Calculated molecular weight 355 aa, 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC026015
Gene Symbol HNRNPD
Gene ID (NCBI) 3184
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14103
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region (PMID:8578590,12704645). AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).