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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HNRNPAB Polyclonal antibody (31175-1-AP)

    WB analysis using 31175-1-AP (same clone as 31175-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31175-1-AP (HNRNPAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31175-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using HNRNPAB Polyclonal antibody (31175-1-AP)

    IHC staining of mouse liver using 31175-1-AP (same clone as 31175-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31175-1-AP (HNRNPAB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPAB Polyclonal antibody (31175-1-AP)

    IF Staining of HeLa using 31175-1-AP (same clone as 31175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPAB antibody (31175-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using HNRNPAB Polyclonal antibody (31175-1-AP)

    IF Staining of Jurkat using 31175-1-AP (same clone as 31175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HNRNPAB antibody (31175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31175-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using HNRNPAB Polyclonal antibody (31175-1-AP)

    IF Staining of Jurkat using 31175-1-AP (same clone as 31175-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HNRNPAB antibody (31175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31175-1-PBS in a different storage buffer formulation.

HNRNPAB Polyclonal antibody, PBS Only

HNRNPAB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31175-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HNRPAB, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1, ABBP1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31175-1-PBS targets HNRNPAB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35010

Product name: Recombinant human HNRNPAB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-62 aa of BC036708

Sequence: MSEAGEEQPMETTGATENGHEAVPEGESPAGAGTGAAAGAGGATAAPPSGNQNGAEGDQINA

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A/B
Calculated molecular weight36 kDa
Observed molecular weight36-40 kDa
GenBank accession numberBC036708
Gene Symbol HNRNPAB
Gene ID (NCBI) 3182
RRIDAB_3669882
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99729
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Heterogeneous nuclear ribonucleoprotein AB (HNRNPAB) is a member of the hnRNP family involved in multiple forms of transcriptional control. It plays an important role in normal biological processes and tumor development and progression. HNRNPAB acts as a direct transcriptional target of c-Myc and promotes G1/S cell cycle progression and cell proliferation via CDK4(PMID: 36657708).

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