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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of Jurkat using 25142-1-AP (same clone as 25142-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of mouse liver using 25142-1-AP (same clone as 25142-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of mouse lung using 25142-1-AP (same clone as 25142-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    WB analysis of HeLa using 25142-1-AP (same clone as 25142-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25142-1-AP (HNRNPA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IHC staining of human colon using 25142-1-AP (same clone as 25142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IHC staining of human stomach using 25142-1-AP (same clone as 25142-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25142-1-AP (HNRNPA3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IF Staining of mouse colon using 25142-1-AP (same clone as 25142-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200, E-cadherin antibody (20874-1-AP, Magenta), CoraLite®594 smooth muscle actin antibody (CL594-14395, red), CD45 antibody (80297-1-RR, Clone: 6O19, orange). This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPA3 Polyclonal antibody (25142-1-AP)

    IF Staining of HeLa using 25142-1-AP (same clone as 25142-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPA3 antibody (25142-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25142-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HNRNPA3 Polyclonal antibody, PBS Only

HNRNPA3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 25142-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

HNRPA3, HNRNPA 3, hnRNP A3, FBRNP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25142-1-PBS targets HNRNPA3 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18248

Product name: Recombinant human HNRNPA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 207-330 aa of BC113470

Sequence: MQSAGSQRGRGGGSGNFMGRGGNFGGGGGNFGRGGNFGGRGGYGGGGGGSRGSYGGGDGGYNGFGGDGGNYGGGPGYSSRGGYGGGGPGYGNQGGGYGGGGGYDGYNEGGNFGGGNYGGGGNYN

相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein A3
Calculated molecular weight 378 aa, 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC113470
Gene Symbol HNRNPA3
Gene ID (NCBI) 220988
RRIDAB_2879921
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP51991
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HNRNPA3 also named as hnRNP A3 or heterogeneous nuclear ribonucleoprotein A3 is a 378 amino acid protein, which contains 2 RRM domains and localizes in nucleus. HNRNPA3 is a component of the spliceosome C complex and play a role in cytoplasmic trafficking of RNA. Binds to the cis-acting response element.

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